el4 mouse lymphoma cell lines  (ATCC)

Journal: Molecular cancer therapeutics

Article Title: STAT5 activation enhances adoptive therapy combined with peptide vaccination by preventing PD-1 inhibition

doi: 10.1158/1535-7163.MCT-24-0505

Figure Lengend Snippet: A ) CD45.1 mice were adoptively transferred with 1–2 × 10 5 gp100+ CD8 T cells co-transduced −CA-STAT5 or +CA-STAT5 and vaccinated with TriVax on the following day (n=3 mice/group). 7 days later, the mice were sacrificed and splenocytes were harvested for cytokine analysis. B ) Representative images demonstrating the percentage of splenocytes producing IFNγ, TNFα, and Granzyme B (GzmB) in response to gp100 peptide stimulation compared to irrelevant (ova) peptide. The number of cells from each group incubated with peptide were normalized based on tetramer staining so that each well had the same number of gp100 tetramer+ T cells. Summary of results depicted in bar graph. C ) Measurement of IFNγ production in an ELISpot assay. The number of CD8 T cells in each group per well were unsorted and normalized to the same number of total gp100 tetramer+ CD8 T cells so that each well had the same number of gp100+ CD8 T cells. D ) CD8 T cells isolated from the experiments described in A were co-cultured with EL4, EL4 pulsed with gp100 short peptide (positive control), or B16F10 tumor cells. Supernatant was harvested after overnight culture from triplicate wells and evaluated for IFNγ production using ELISA. E ) Cytotoxic killing potential was measured by co-culturing +CA-STAT5 or −CA-STAT5 CD8 T cells with CFSE-labeled EL4, EL4 pulsed with gp100 short peptide, or B16F10 at an effector-to-target ratio (gp100 tetramer+ CD8 T cells to B16 melanoma) of 10:1. Summary graphs of at least two independent experiments from each figure is shown.

Article Snippet: Mouse lymphoma cell line EL4 expressing mouse MHC-I (Db/Kb) was purchased from the American Type Culture Collection (2015) and maintained as recommended by the vendor.

Techniques: Incubation, Staining, Enzyme-linked Immunospot, Isolation, Cell Culture, Positive Control, Enzyme-linked Immunosorbent Assay, Labeling

Journal: Scientific Reports

Article Title: ITK overexpression enhances T cell cytotoxicity against DLBCL through the TCR-Ca 2+ -Calcineurin-NFAT-IFN-γ pathway

doi: 10.1038/s41598-025-16112-3

Figure Lengend Snippet: Effects of Itk -overexpressing CTLL-2 cells on A20. ( a ) Schematic diagram of the direct co-culture model of CTLL-2 cells ( Itk OE) and A20 mouse B-cell lymphoma cells. ( b , c ). Effects of Itk overexpression in CTLL-2 cells on the proliferation of A20 cells. Scale bar, 50 µm. ( d , e ). Effects of Itk -overexpressing CTLL-2 cells on the apoptosis of A20 cells. Scale bar, 50 µm. ( f ) Fitted curve of cell survival rates in the rituximab synergistic experiment. ( g ) Bar chart of cell survival rates in each group in the rituximab synergistic experiment. n = 6, ** p < 0.01, *** p < 0.001, **** p < 0.0001, two-tailed t test.

Article Snippet: The mouse cytotoxic T lymphocyte line CTLL-2 was purchased from the American Type Culture Collection (ATCC, USA, TIB-214), and the mouse B-cell lymphoma cell line A20 was purchased from ATCC (USA, TIB-208).

Techniques: Co-Culture Assay, Over Expression, Two Tailed Test